Vitamin B

Vitamin B 6 is a water-soluble vitamin and is part of the vitamin B complex group. Several forms of the vitamin are known, but pyridoxal phosphate (PLP) is the active form and is a cofactor in many reactions of amino acid metabolism, including transamination, deamination, and decarboxylation. PLP also is necessary for the enzymatic reaction governing the release of glucose from glycogen .

Vitamin B 6 is a water-soluble compound that was discovered in the 1930s during nutrition studies on rats. In 1934, a Hungarian physician, Paul György discovered a substance that was able to cure a skin disease in rats (dermititis acrodynia), this substance he named vitamin B 6 .In 1938, Samuel Lepkovsky isolated vitamin B 6 from rice bran. Harris and Folkers in 1939 determined the structure of pyridoxine, and, in 1945, Snell was able to show that there are two forms of vitamin B 6 , pyridoxal and pyridoxamine. Vitamin B 6 was named pyridoxine to indicate its structural homology to pyridine . All three forms of vitamin B 6 are precursors of an activated compound known as pyridoxal 5'-phosphate (PLP), which plays a vital role as the co-factor of a large number of essential enzymes in the human body.

Enzymes dependent on PLP focus a wide variety of chemical reactions mainly involving amino acids. The reactions carried out by the PLP-dependent enzymes that act on amino acids include transfer of the amino group, decarboxylation, racemization, and beta- or gamma-elimination or replacement. Such versatility arises from the ability of PLP to covalently bind the substrate, and then to act as an electrophilic catalyst, thereby stabilizing different types of carbanionic reaction intermediates.

Overall, the Enzyme Commission has catalogued more than 140 PLP-dependent activities, corresponding to ~4% of all classified activities.

Seven forms of this vitamin are known:

All forms except PA can be interconverted.

Pyridoxal phosphate, the metabolically active form of vitamin B 6 , is involved in many aspects of macronutrient metabolism, neurotransmitter synthesis, histamine synthesis, hemoglobin synthesis and function and gene expression . Pyridoxal phosphate generally serves as a coenzyme for many reactions and can help facilitate decarboxylation, transamination, racemization, elimination, replacement and beta-group interconversion reactions.The liver is the site for vitamin B 6 metabolism.

Pyridoxal phosphate (PLP) is a cofactor in transaminases that can catabolize amino acids. PLP is also an essential component of two enzymes that converts methionine to cysteine via two reactions. Low vitamin B 6 status will result in decreased activity of these enzymes. PLP is also an essential cofactor for enzymes involved in the metabolism of selenomethionine to selenohomocysteine and then from selenohomocysteine to hydrogen selenide . Vitamin B 6 is also required for the conversion of tryptophan to niacin and low vitamin B 6 status will impair this conversion.PLP is also used to create physiologically active amines by decarboxylation of amino acids. Some notable examples of this include: histidine to histamine , tryptophan to serotonin , glutamate to gamma-aminobutyric acid (GABA), and dihydroxyphenylalanine to dopamine .

Vitamin B 6 also plays a role in gluconeogenesis . Pyridoxal phosphate can catalyze transamination reactions that are essential for the providing amino acids as a substrate for gluconeogenesis. Also, vitamin B 6 is a required coenzyme of glycogen phosphorylase,the enzyme that is necessary for glycogenolysis to occur.

Vitamin B 6 is an essential component of enzymes that facilitate the biosynthesis of sphingolipids .Particularly, the synthesis of ceramide requires PLP. In this reaction serine is decarboxylated and combined with palmitoyl-CoA to form sphinganine which is combined with a fatty acyl CoA to form dihydroceramide. Dihydroceramide is then further desaturated to form ceramide. In addition, the breakdown of sphingolipids is also dependent on vitamin B 6 since S1P lyase, the enzyme responsible for breaking down sphingosine-1-phosphate, is also PLP dependent.

Pyridoxal phosphate is involved in almost all amino acid metabolism, from synthesis to breakdown.

1. Transamination: transaminase enzymes needed to break down amino acids are dependent on the presence of pyridoxal phosphate. The proper activity of these enzymes are crucial for the process of moving amine groups from one amino acid to another.

2. Transsulfuration: Pyridoxal phosphate is a coenzyme needed for the proper function of the enzymes cystathionine synthase and cystathionase. These enzymes work to transform methionine into cysteine.

3. Selenoamino acid metabolism: Selenomethionine is the primary dietary form of selenium. Pyridoxal phosphate is needed as a cofactor for the enzymes that allow selenium to be used from the dietary form. Pyridoxal phosphate also plays a cofactor role in releasing selenium from selenohomocysteine to produce hydrogen selenide. This hydrogen selenide can then be used to incorporate selenium into selenoproteins.

4. Vitamin B 6 is also required for the conversion of tryptophan to niacin and low vitamin B 6 status will impair this conversion.

Pyridoxal phosphate-dependent enzymes play a role in the biosynthesis of four important neurotransmitters : serotonin , epinephrine , norepinephrine and gamma-aminobutyric acid .Serine racemase, which synthesizes the neuromodulator D-serine, is also a pyridoxal phosphate-dependent enzyme.

The Institute of Medicine notes that "No adverse effects associated with Vitamin B 6 from food have been reported. This does not mean that there is no potential for adverse effects resulting from high intakes. Because data on the adverse effects of Vitamin B 6 are limited, caution may be warranted. Sensory neuropathy has occurred from high intakes of supplemental forms."See the full Dietary Reference Intake Table [1] from the Institute of Medicine.

Vitamin B 6 is widely distributed in foods in both its free and bound forms. Good sources include meats , whole grain products, vegetables , nuts and bananas . Cooking, сторидж and processing losses of vitamin B 6 vary and in some foods may be more than 50%,depending on the form of vitamin present in the food. Plant foods lose the least during processing as they contain mostly pyridoxine which is far more stable than the pyridoxal or pyridoxamine found in animal foods. For example, milk can lose 30-70% of its vitamin B 6 content when dried .Vitamin B 6 is found in the germ and aleurone layer of grains and milling results to the reduction of this vitamin in white flour. Freezing and canning are other food processing methods that results in the loss of vitamin B 6 in foods.

Vitamin B 6 is absorbed in the jejunum and ileum via passive diffusion. With the capacity for absorption being so great, animals are able to absorb quantities much greater than what is needed for physiological demands. The absorption of pyridoxal phosphate and pyridoxamine phosphate involves their dephosphorylation catalyzed by a membrane-bound alkaline phosphatase . Those products and non-phosphorylated vitamers in the digestive tract are absorbed by diffusion, which is driven by trapping of the vitamin as 5'-phosphates through the action of phosphorylation (by a pyridoxal kinase) in the jejunal mucosa. The trapped pyridoxine and pyridoxamine are oxidized to pyridoxal phosphate in the tissue.

The products of vitamin B 6 metabolism are excreted in the urine; the major product of which is 4-pyridoxic acid. It has been estimated that 40-60% of ingested vitamin B 6 is oxidized to 4-pyridoxic acid. Several studies have shown that 4-pyridoxic acid is undetectable in the urine of vitamin B 6 deficient subjects, making it a useful clinical marker to assess the vitamin B 6 status of an individual.Other products of vitamin B 6 metabolism that are excreted in the urine when high doses of the vitamin have been given include pyridoxal, pyridoxamine, and pyridoxine and their phosphates. A small amount of vitamin B 6 is also excreted in the feces.

While severe vitamin B 6 deficiency results in dermatologic and neurologic changes, less severe cases present with metabolic lesions associated with insufficient activities of the coenzyme pyridoxal phosphate . The most prominent of the lesions is due to impaired tryptophan - niacin conversion. This can be detected based on urinary excretion of xanthurenic acid after an oral tryptophan load. Vitamin B 6 deficiency can also result from impaired transsulfuration of methionine to cysteine . The pyridoxal phosphate-dependent transaminases and glycogen phosphorylase provide the vitamin with its role in gluconeogenesis , so deprivation of vitamin B 6 results in impaired glucose tolerance.

A deficiency of vitamin B 6 alone is relatively uncommon and often occurs in association with other vitamins of the B complex. The elderly and alcoholics have an increased risk of vitamin B 6 deficiency, as well as other micronutrient deficiencies.Renal patients undergoing dialysis may experience vitamin B 6 deficiency. Also, patients with liver disease, rheumatoid arthritis and those infected with HIV also appear to be at risk, despite adequate dietary intakes.The availability of vitamin B 6 to the body can be affected by certain drugs such as anticonvulsants and corticosteroids .The drug isoniazid (used in the treatment of tuberculosis), and cycloserine, penicillamine, and hydrocortisone all interfere with vitamin B 6 metabolism. These drugs may form a complex with vitamin B 6 that is inhibitory for потрясавшему or they may positively displace PLP from binding sites.

The biochemical assessment of vitamin B 6 status is essential, as the clinical signs and symptoms of vitamin B 6 deficiency are very nonspecific.The three biochemical tests most widely used are the activation coefficient for the erythrocyte enzyme aspartate aminotransferase, plasma pyridoxal phosphate (PLP) concentrations, and the urinary excretion of vitamin B 6 degradation products, specifically urinary pyridoxic acid. Of these, plasma PLP is probably the best single measure because it reflects tissue stores.When plasma pyridoxal phosphate is less than 10nmol/L, it is indicative of vitamin B 6 deficiency.Urinary 4-pyridoxic acid is also an indicator of vitamin B 6 deficiency. Urinary 4-pyridoxic of less than 3.0 mmol/day is suggestive of vitamin B 6 deficiency.

Adverse effects have only been documented from vitamin B 6 supplements and never from food sources. This article only discusses the safety of the common supplemental form of vitamin B 6 pyridoxine (for a full discussion please see pyridoxine ). Toxicologic animal studies identify specific destruction of the dorsal root ganglia which is documented in human cases of overdosage of pyridoxine.Although vitamin B 6 is a water-soluble vitamin and is excreted in the urine, doses of pyridoxine in excess of the RDI over long periods of time thus result in painful and ultimately irreversible neurological problems.

The primary symptoms are pain and numbness of the extremities, and in severe cases difficulty walking. Sensory neuropathy typically develops at doses of pyridoxine in excess of 1,000 mg per day. However, there have been a few case reports of individuals who developed sensory neuropathies at doses of less than 500 mg daily over a period of months. None of the studies, in which an objective neurological examination was performed, found evidence of sensory nerve damage at intakes of pyridoxine below 200 mg/day. This condition is usually reversible when supplementation is stopped.

Existing authorisations and valuations vary considerably worldwide. In 1993 the European Community Scientific Committee on Food defines intakes of 50 mg vitamin B6 per day as harmful and established tolerable upper intake level of 25 mg/day for adults in 2000.

The Expert Group on Vitamins and Minerals of the Food Standard Agency UK (UK EVM) derived a safe upper level (SUL) of 10 mg/day for a 60 kg adult in 2003.

The tolerable upper limit has been set by the US FDA at 100 mg/day in 2000.The nutrient reference values in Australia and New Zealand recommend an upper limit of 50 mg a day in adults. "The same figure was set for pregnancy and lactation as there is no evidence of teratogenicity at this level. The UL was set based on metabolic body size and growth considerations for all other ages and life stages except infancy. It was not possible to set a UL for infants, so intake is recommended in the form of food, milk or formula." "The ULs were set using results of studies involving long-term oral administration of pyridoxine at doses of less than 1g/day (Berger & Schaumburg 1984, Bernstein & Lobitz 1988, Dalton 1985, Dalton & Dalton 1987, Del Tredici et al 1985, FNB:IOM 1998, Parry & Bredesen 1985). A NOAEL of 200 mg/day was identified from the studies of Bernstein & Lobitz (1988) and Del Tredici et al (1985). These studies involved subjects who had generally been on the supplements for 5 to 6 months or less. The study of Dalton and Dalton (1987), however, suggested that symptoms might take substantially longer than this to appear. In this latter retrospective survey, subjects who reported symptoms had been on supplements for 2.9 years on average. Those reporting no symptoms had taken supplements for 1.9 years."

Because there have been no placebo-controlled studies showing therapeutic benefits of high doses of pyridoxine, and the well documented occurrence of significant toxic effects there is little reason to exceed the RDI using supplements unless under medical supervision e.g. in treatment of primary hyperoxaluria .

Vitamin B 6 has been used to treat nausea and vomiting in early pregnancy for decades, commonly in conjunction with other medications such as metoclopramide or doxylamine. Alone, it has been found safe and effective, though any woman's prenatal caregiver must help guide treatment for these symptoms.

At least one preliminary study has found that this vitamin may increase dream vividness or the ability to recall dreams.It is thought that this effect may be due to the role this vitamin plays in the conversion of tryptophan to serotonin .

The intake of vitamin B 6 , from either diet or supplements, could cut the risk of Parkinson's disease by half according to a prospective study from the Netherlands. "Stratified analyses showed that this association was restricted to smokers," wrote the authors.

Pyridoxine has a role in preventing heart disease. Without enough pyridoxine, a compound called homocysteine builds up in the body. Homocysteine damages blood vessel linings, setting the stage for plaque buildup when the body tries to heal the damage. Vitamin B 6 prevents this buildup, there...